The mutation was highly associated

The mutation was highly associated with coat length in these animals (genotypic test assuming dominance, P=7.3 1020), and when considered as a binary trait comprising 42 cases and 40 controls (1=slick, >1=not slick), the mutation segregated in 79 of 82 individuals (genotypic test assuming dominance, P=4.7 1017). Haplotype based analysis was then conducted using 25 Illumina SNP50 BeadChip SNPs in a 1 Mbp consensus slick interval reported in independent analyses of Senepol16 and Senepol crossbreeds17. Notably, haplotypes of the third nonsegregating animal did not share an obvious lineage with the 229 kb contiguous block found in all other slick coded animals, making the existence of an alternative, hidden causative mutation shared by all slick coded animals unlikely.Exome sequence analysisTo look for alternative mutations at the slick locus, we next obtained exome sequence data from 115 animals representing Senepol, Angus, Belgian Blue, Brahman, Charolais, Holstein Friesian, Jersey, Nelore, Simmental and Yak breeds.

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